Bayern Munich trio Bastian Schweinsteiger, Toni Kroos and Jerome Boateng will miss Germany's friendly clash with the Netherlands next week.
Borussia Dortmund's Marcel Schmelzer will also be sidelined for Wednesday's match in Amsterdam.
Experienced midfielder Schweinsteiger and young playmaker Kroos are suffering from infections, while Boateng picked up a hamstring injury against Eintracht Frankfurt on Saturday and Schmelzer has a bruised ankle.
Elsewhere, Schalke defensive midfielder Roman Neustadter has expressed his delight at being called up by national team coach Joachim Loew for the first time.
"At first I was shocked, because I didn't expect it," the 24-year-old told Bundesliga.de. "Of course I was extremely delighted and could not find words for the joy I felt.
"(Loew) said that I deserve it due to my performance, he now would like to get to know me and that he is looking forward to Monday."