Juventus coach Antonio Conte has told his players they can never give any less than 100 percent on the pitch after their 1-0 loss to Roma.
Conte revealed that none of his charges had complained of fatigue prior to the Stadio Olimpico trip, with reports he intends to make them train at 8am on Tuesday.
"I spoke clearly to the boys before the game against Roma," he said after Saturday's match.
"I told them who you feel tired or rundown from the (Champions League) game, tell me right away, there will be no problems.
"I mean, I'm open to the team, and no one is turned away. On Saturday night, I was not told by anyone about any problems.
"If you tell the coach that you are ready, then you have to prove it on the field, spitting blood, regardless of how good performance you put in."
The Bianconeri's next clash is at home against Conte's former club, Siena, on Sunday.