Antonio Conte's side were dominant in the league last season, finishing nine points clear of Napoli as the Turin club secured back-to-back titles.
Juve will start the 2013-14 campaign against a Sampdoria side who were the only team to beat them twice in Serie A last season.
Conte has been busy during the transfer window, adding the likes of Carlos Tevez, Fernando Llorente and Angelo Ogbonna to his squad.
The first round of Serie A fixtures, which are due to take place on the weekend of August 24, will see last season's runners-up Napoli entertain Bologna, while Milan are on the road at new-boys Verona and Inter welcome Genoa to San Siro.
Fiorentina and Lazio have been handed home games against Catania and Udinese respectively, Parma play host to Chievo and Atalanta face Cagliari in Sardinia.
Last season's Serie B champions Sassuolo travel to Torino and Livorno start life back in the top tier of Italian football at home against Roma.