Captain Moussa Maazou leads the way, in a squad comprising few local players - unlike the previous edition - while two players in striker Modibo Sidibe and midfielder Lacina were included as free agents.
Three players - Mohammed Wonkoye, Idi Sabiou and Ibrahim Benjamen - were dropped from the preliminary list.
Niger are in Group B alongside Ghana, Mali and the DR Congo.
Meanwhile, Tunisia coach Sami Trabelsi named his squad for the tournament, dropping Atletico Madrid midfielder Kader Oueslati and Rami Bedoui of Etoile du Sahel from his initial group of 25 players.
Wahbi Khazri of French Ligue 1 side Bastia made the list amid rumours that he would be dropped following injury issues.
Tunisia are drawn against Cote d'Ivoire, Togo and fellow North Africans Algeria in Group D.