Liverpool boss Brendan Rodgers says Martin Skrtel is 'highly unlikely' to feature in the club's Europa League tie against Zenit St Petersburg.
The Slovakia international was an unused substitute in Liverpool's 5-0 win over Swansea in the Premier League on Sunday.
And a knee injury looks set to rule him out of the second-leg tie against the Russian champions on Thursday.
"Skrtel is being assessed," Rodgers said. "I think he'll struggle with his knee, he had a knock on it and he tried to train and made it worse. It's highly unlikely he'll play."
The injury to Skrtel means Jamie Carragher and Daniel Agger are expected to continue in the centre of defence against Zenit.
Elsewhere, injury-plagued striker Fabio Borini is set to miss up to two months of the season after sustaining a dislocated shoulder in Sunday's win against Swansea.
"By the weekend, he'll look to have an operation and you're then looking at six to eight weeks," Rodgers said.
"It's been a difficult season for Fabio, coming into such a big club, getting injured early on, coming back and then working hard for his moment and doing his shoulder.
"It's been difficult for him but he's a tough-minded young boy and he'll get through the op well and we'll see how he recovers."