Nacional Asuncion remain well clear atop the Paraguay Division Profesional despite a shock 1-0 loss to Sportivo Luqueno on Saturday.
Gustavo Morinigo's men are still set to claim the title, sitting 13 points ahead of second-placed Guarani with five matches to play.
But their defeat at the hands of bottom side Sportivo Luqueno was a surprise, with Luis Mino's 57th-minute penalty the difference between the teams.
It was Sportivo's second straight win and just their third of the campaign, and they remain bottom – level on points with 11th-placed Rubio Nu, who have played a game less.
Guarani kept the title race alive with a 4-0 thrashing of Sol de America. Rodrigo Teixeira netted a brace while Marcelo Palau and Derlis Gonzalez also got on the scoresheet in the convincing win.
Cerro Porteno slipped to third on 30 points after being held to a 1-1 draw at home to Olimpia.
Oscar Romero's 50th-minute opener was cancelled out by Olimpia defender Julio Manzur and the hosts were reduced to 10 men late on when Nestor Ramirez saw red.
Elsewhere, Rubio Nu came from behind to salvage a 1-1 draw against General Diaz, while a Dionisio Perez brace saw Deportivo Capiata beat Deportivo Carapegua 2-0.
Libertad were 3-2 winners at Cerro Porteno PF, setting their victory up with a three-goal first half.